Nonenzymatic ligation of DNA with a reversible step and a final linkage that can be used in PCR.

TitleNonenzymatic ligation of DNA with a reversible step and a final linkage that can be used in PCR.
Publication TypeJournal Article
Year of Publication2012
AuthorsEngelhart, AE, Cafferty, BJ, C Okafor, D, Chen, MC, Williams, LDean, Lynn, DG, Hud, NV
JournalChemBioChem
Volume13
Issue8
Pagination1121-4
Date Published2012 May 29
ISSN1439-7633
KeywordsCombinatorial Chemistry Techniques, DNA, DNA Probes, DNA-Directed DNA Polymerase, Morpholines, Polymerase Chain Reaction
Abstract

Nonenzymatic DNA ligation chemistries containing a reversible step allow thermodynamic control of product formation, but they are not necessarily compatible with polymerase enzymes. We report a ligation system that uses commercially available reagents, includes a reversible step, and results in a linkage that can function as a template for PCR amplification with accurate sequence transfer.
DOI10.1002/cbic.201200167
Alternate JournalChembiochem
PubMed ID22556064